Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction

We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidig...

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Dettagli Bibliografici
Pubblicato in:Genome Biol
Autori principali: Genshaft, Alex S, Li, Shuqiang, Gallant, Caroline J., Darmanis, Spyros, Prakadan, Sanjay M., Ziegler, Carly G. K., Lundberg, Martin, Fredriksson, Simon, Hong, Joyce, Regev, Aviv, Livak, Kenneth J., Landegren, Ulf, Shalek, Alex K.
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2016
Soggetti:
Method
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5027636/
https://ncbi.nlm.nih.gov/pubmed/27640647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-1045-6
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