Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction

We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidig...

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發表在:Genome Biol
Main Authors: Genshaft, Alex S, Li, Shuqiang, Gallant, Caroline J., Darmanis, Spyros, Prakadan, Sanjay M., Ziegler, Carly G. K., Lundberg, Martin, Fredriksson, Simon, Hong, Joyce, Regev, Aviv, Livak, Kenneth J., Landegren, Ulf, Shalek, Alex K.
格式: Artigo
語言:Inglês
出版: BioMed Central 2016
主題:
Method
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5027636/
https://ncbi.nlm.nih.gov/pubmed/27640647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-1045-6
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