Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction

We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidig...

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Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Genshaft, Alex S, Li, Shuqiang, Gallant, Caroline J., Darmanis, Spyros, Prakadan, Sanjay M., Ziegler, Carly G. K., Lundberg, Martin, Fredriksson, Simon, Hong, Joyce, Regev, Aviv, Livak, Kenneth J., Landegren, Ulf, Shalek, Alex K.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5027636/
https://ncbi.nlm.nih.gov/pubmed/27640647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-016-1045-6
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