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Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed...

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Bibliographic Details
Main Authors: Ke, Rongqin, Nong, Rachel Yuan, Fredriksson, Simon, Landegren, Ulf, Nilsson, Mats
Format: Artigo
Language:Inglês
Published: Public Library of Science 2013
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3713053/
https://ncbi.nlm.nih.gov/pubmed/23874999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069813
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