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Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed...

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Detalhes bibliográficos
Main Authors: Ke, Rongqin, Nong, Rachel Yuan, Fredriksson, Simon, Landegren, Ulf, Nilsson, Mats
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3713053/
https://ncbi.nlm.nih.gov/pubmed/23874999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069813
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