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Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed...

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Autores principales: Ke, Rongqin, Nong, Rachel Yuan, Fredriksson, Simon, Landegren, Ulf, Nilsson, Mats
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2013
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3713053/
https://ncbi.nlm.nih.gov/pubmed/23874999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069813
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