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Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed...

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Bibliografiske detaljer
Main Authors: Ke, Rongqin, Nong, Rachel Yuan, Fredriksson, Simon, Landegren, Ulf, Nilsson, Mats
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3713053/
https://ncbi.nlm.nih.gov/pubmed/23874999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069813
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