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Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed...

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Autori principali: Ke, Rongqin, Nong, Rachel Yuan, Fredriksson, Simon, Landegren, Ulf, Nilsson, Mats
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3713053/
https://ncbi.nlm.nih.gov/pubmed/23874999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069813
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