Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells

Translation is an essential step in gene expression. In this study, we used an improved SunTag system to label nascent proteins and image translation of single messenger ribonucleoproteins (mRNPs) in human cells. Using a dedicated reporter RNA, we observe that translation of single mRNPs stochastica...

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Détails bibliographiques
Publié dans:J Cell Biol
Auteurs principaux: Pichon, Xavier, Bastide, Amandine, Safieddine, Adham, Chouaib, Racha, Samacoits, Aubin, Basyuk, Eugenia, Peter, Marion, Mueller, Florian, Bertrand, Edouard
Format: Artigo
Langue:Inglês
Publié: The Rockefeller University Press 2016
Sujets:
Research Articles
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5021097/
https://ncbi.nlm.nih.gov/pubmed/27597760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201605024
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