Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells

Translation is an essential step in gene expression. In this study, we used an improved SunTag system to label nascent proteins and image translation of single messenger ribonucleoproteins (mRNPs) in human cells. Using a dedicated reporter RNA, we observe that translation of single mRNPs stochastica...

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Detalhes bibliográficos
Publicado no:J Cell Biol
Main Authors: Pichon, Xavier, Bastide, Amandine, Safieddine, Adham, Chouaib, Racha, Samacoits, Aubin, Basyuk, Eugenia, Peter, Marion, Mueller, Florian, Bertrand, Edouard
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 2016
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5021097/
https://ncbi.nlm.nih.gov/pubmed/27597760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201605024
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