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Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants.

Conjugating cells of the ciliate Tetrahymena thermophila were electroporated in the presence of plasmid DNA containing a paromomycin-resistant ribosomal RNA gene (rDNA). Cells were selected with paromomycin following 12-24 hr of growth on nonselective medium. Resistant cells appeared after 2-3 days....

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Autori principali: Gaertig, J, Gorovsky, M A
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1992
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC50092/
https://ncbi.nlm.nih.gov/pubmed/1409625
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