Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants.
Conjugating cells of the ciliate Tetrahymena thermophila were electroporated in the presence of plasmid DNA containing a paromomycin-resistant ribosomal RNA gene (rDNA). Cells were selected with paromomycin following 12-24 hr of growth on nonselective medium. Resistant cells appeared after 2-3 days....
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1992
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50092/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1409625/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.19.9196 |
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