Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants.

Conjugating cells of the ciliate Tetrahymena thermophila were electroporated in the presence of plasmid DNA containing a paromomycin-resistant ribosomal RNA gene (rDNA). Cells were selected with paromomycin following 12-24 hr of growth on nonselective medium. Resistant cells appeared after 2-3 days....

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Vydáno v:	Proc Natl Acad Sci U S A
Hlavní autoři:	Gaertig, J, Gorovsky, M A
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	National Academy of Sciences 1992
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50092/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1409625/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.19.9196
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