Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants.
Conjugating cells of the ciliate Tetrahymena thermophila were electroporated in the presence of plasmid DNA containing a paromomycin-resistant ribosomal RNA gene (rDNA). Cells were selected with paromomycin following 12-24 hr of growth on nonselective medium. Resistant cells appeared after 2-3 days....
Spremljeno u:
| Izdano u: | Proc Natl Acad Sci U S A |
|---|---|
| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1992
|
| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC50092/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1409625/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.19.9196 |
| Oznake: |
Bez oznaka, Budi prvi tko označuje ovaj zapis!
|