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Retroviral Transduction of Murine Primary T Lymphocytes
In comparison to human T cells, efficient retroviral gene transfer and subsequent expansion of murine primary T cells is more difficult to achieve. Herein, we describe an optimized gene transfer protocol utilizing an ecotropic viral vector to transduce primary murine T cells activated with magnetic...
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| Foilsithe in: | Methods Mol Biol |
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| Main Authors: | , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
2009
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5003426/ https://ncbi.nlm.nih.gov/pubmed/19110621 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-409-4_7 |
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