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Retroviral Transduction of Murine Primary T Lymphocytes

In comparison to human T cells, efficient retroviral gene transfer and subsequent expansion of murine primary T cells is more difficult to achieve. Herein, we describe an optimized gene transfer protocol utilizing an ecotropic viral vector to transduce primary murine T cells activated with magnetic...

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Publicat a:Methods Mol Biol
Autors principals: Lee, James, Sadelain, Michel, Brentjens, Renier
Format: Artigo
Idioma:Inglês
Publicat: 2009
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5003426/
https://ncbi.nlm.nih.gov/pubmed/19110621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-409-4_7
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