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Monobody-mediated alteration of enzyme specificity
Current methods for engineering enzymes modify enzymes themselves and require a detailed mechanistic understanding or a high-throughput assay. Here, we describe a new approach where catalytic properties are modulated with synthetic binding proteins, termed monobodies, directed to an unmodified enzym...
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| Udgivet i: | Nat Chem Biol |
|---|---|
| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4989918/ https://ncbi.nlm.nih.gov/pubmed/26322825 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.1896 |
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