Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP

Predictable, clean genetic modification (GM) in livestock is important for reliable phenotyping and biosafety. Here we reported the generation of isozygous, functional myostatin (MSTN) knockout cloned pigs free of selectable marker gene (SMG) by CRISPR/Cas9 and Cre/LoxP. CRISPR/Cas9-mediated homolog...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Bi, Yanzhen, Hua, Zaidong, Liu, Ximei, Hua, Wenjun, Ren, Hongyan, Xiao, Hongwei, Zhang, Liping, Li, Li, Wang, Zhirui, Laible, Götz, Wang, Yan, Dong, Faming, Zheng, Xinmin
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2016
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4987667/
https://ncbi.nlm.nih.gov/pubmed/27530319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep31729
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