Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP

Predictable, clean genetic modification (GM) in livestock is important for reliable phenotyping and biosafety. Here we reported the generation of isozygous, functional myostatin (MSTN) knockout cloned pigs free of selectable marker gene (SMG) by CRISPR/Cas9 and Cre/LoxP. CRISPR/Cas9-mediated homolog...

Description complète

Enregistré dans:
Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Bi, Yanzhen, Hua, Zaidong, Liu, Ximei, Hua, Wenjun, Ren, Hongyan, Xiao, Hongwei, Zhang, Liping, Li, Li, Wang, Zhirui, Laible, Götz, Wang, Yan, Dong, Faming, Zheng, Xinmin
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2016
Sujets:
Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4987667/
https://ncbi.nlm.nih.gov/pubmed/27530319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep31729
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!

Documents similaires