Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP

Predictable, clean genetic modification (GM) in livestock is important for reliable phenotyping and biosafety. Here we reported the generation of isozygous, functional myostatin (MSTN) knockout cloned pigs free of selectable marker gene (SMG) by CRISPR/Cas9 and Cre/LoxP. CRISPR/Cas9-mediated homolog...

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Bibliografske podrobnosti
izdano v:Sci Rep
Main Authors: Bi, Yanzhen, Hua, Zaidong, Liu, Ximei, Hua, Wenjun, Ren, Hongyan, Xiao, Hongwei, Zhang, Liping, Li, Li, Wang, Zhirui, Laible, Götz, Wang, Yan, Dong, Faming, Zheng, Xinmin
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2016
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4987667/
https://ncbi.nlm.nih.gov/pubmed/27530319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep31729
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