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Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping
We have developed a method to measure strong transmembrane (TM) helix interaction affinities in lipid bilayers that are difficult to measure by traditional dilution methods. The method, called steric trapping, couples dissociation of biotinylated TM helices to a competitive binding by monovalent str...
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| izdano v: | Methods Mol Biol |
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| Main Authors: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2013
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4986992/ https://ncbi.nlm.nih.gov/pubmed/23975771 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-583-5_3 |
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