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Method to measure strong protein–protein interactions in lipid bilayers using a steric trap
Measuring high affinity protein–protein interactions in membranes is extremely challenging because there are limitations to how far the interacting components can be diluted in bilayers. Here we show that a steric trap can be employed for stable membrane interactions. We couple dissociation to a com...
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| Hlavní autoři: | , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
2010
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2993359/ https://ncbi.nlm.nih.gov/pubmed/21041662 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1010348107 |
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