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Method to measure strong protein–protein interactions in lipid bilayers using a steric trap

Measuring high affinity protein–protein interactions in membranes is extremely challenging because there are limitations to how far the interacting components can be diluted in bilayers. Here we show that a steric trap can be employed for stable membrane interactions. We couple dissociation to a com...

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Hlavní autoři: Hong, Heedeok, Blois, Tracy M., Cao, Zheng, Bowie, James U.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2993359/
https://ncbi.nlm.nih.gov/pubmed/21041662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1010348107
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