Evaluation of an Hprt-Luciferase Reporter Gene on a Mammalian Artificial Chromosome in Response to Cytotoxicity

BACKGROUND: Hypoxanthine guanine phosphoribosyltransferase (Hprt) is known as a house-keeping gene, and has been used as an internal control for real-time quantitative RT-PCR and various other methods of gene expression analysis. To evaluate the Hprt mRNA levels as a reference standard, we engineere...

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Bibliographic Details
Published in:Yonago Acta Med
Main Authors: Endo, Takeshi, Noda, Natsumi, Kuromi, Yasushi, Kokura, Kenji, Kazuki, Yasuhiro, Oshimura, Mitsuo, Ohbayashi, Tetsuya
Format: Artigo
Language:Inglês
Published: Tottori University Faculty of Medicine 2016
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Original Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4973025/
https://ncbi.nlm.nih.gov/pubmed/27493490
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