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Primer Extension Reactions for the PCR- based α- complementation Assay

The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity of the poliovirus polymerase 3D-pol (DeStefano, 2010...

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Bibliografiska uppgifter
I publikationen:Bio Protoc
Huvudupphovsmän: Achuthan, Vasudevan, DeStefano, Jeffrey J.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2015
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4972330/
https://ncbi.nlm.nih.gov/pubmed/27500187
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