Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase using spFRET

Single positioned nucleosomes have been extensively employed as simple model experimental systems for analysis of various intranuclear processes. Here we describe an experimental system containing positioned mononucleosomes allowing transcription by various RNA polymerases. Each DNA template contain...

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Vydáno v:Methods Mol Biol
Hlavní autoři: Kudryashova, Kseniya S., Chertkov, Oleg V., Nikitin, Dmitry V., Pestov, Nikolai A., Kulaeva, Olga I., Efremenko, Anastasija V., Solonin, Alexander S., Kirpichnikov, Mikhail P., Studitsky, Vasily M., Feofanov, Alexey V.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4966549/
https://ncbi.nlm.nih.gov/pubmed/25827893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2474-5_23
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