Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase using spFRET

Single positioned nucleosomes have been extensively employed as simple model experimental systems for analysis of various intranuclear processes. Here we describe an experimental system containing positioned mononucleosomes allowing transcription by various RNA polymerases. Each DNA template contain...

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Pubblicato in:Methods Mol Biol
Autori principali: Kudryashova, Kseniya S., Chertkov, Oleg V., Nikitin, Dmitry V., Pestov, Nikolai A., Kulaeva, Olga I., Efremenko, Anastasija V., Solonin, Alexander S., Kirpichnikov, Mikhail P., Studitsky, Vasily M., Feofanov, Alexey V.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2015
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4966549/
https://ncbi.nlm.nih.gov/pubmed/25827893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2474-5_23
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