Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase using spFRET

Single positioned nucleosomes have been extensively employed as simple model experimental systems for analysis of various intranuclear processes. Here we describe an experimental system containing positioned mononucleosomes allowing transcription by various RNA polymerases. Each DNA template contain...

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Publicado no:Methods Mol Biol
Main Authors: Kudryashova, Kseniya S., Chertkov, Oleg V., Nikitin, Dmitry V., Pestov, Nikolai A., Kulaeva, Olga I., Efremenko, Anastasija V., Solonin, Alexander S., Kirpichnikov, Mikhail P., Studitsky, Vasily M., Feofanov, Alexey V.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4966549/
https://ncbi.nlm.nih.gov/pubmed/25827893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2474-5_23
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