Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase using spFRET

Single positioned nucleosomes have been extensively employed as simple model experimental systems for analysis of various intranuclear processes. Here we describe an experimental system containing positioned mononucleosomes allowing transcription by various RNA polymerases. Each DNA template contain...

Full description

Saved in:
Bibliographic Details
Published in:Methods Mol Biol
Main Authors: Kudryashova, Kseniya S., Chertkov, Oleg V., Nikitin, Dmitry V., Pestov, Nikolai A., Kulaeva, Olga I., Efremenko, Anastasija V., Solonin, Alexander S., Kirpichnikov, Mikhail P., Studitsky, Vasily M., Feofanov, Alexey V.
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4966549/
https://ncbi.nlm.nih.gov/pubmed/25827893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2474-5_23
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items