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Long shelf-life streptavidin support-films suitable for electron microscopy of biological macromolecules
We describe a rapid and convenient method of growing streptavidin (SA) monolayer crystals directly on holey-carbon EM grids. As expected, these SA monolayer crystals retain their biotin-binding function and crystalline order through a cycle of embedding in trehalose and, later, its removal. This fac...
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| Publicado no: | J Struct Biol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4943657/ https://ncbi.nlm.nih.gov/pubmed/27320699 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2016.06.009 |
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