Long shelf-life streptavidin support-films suitable for electron microscopy of biological macromolecules

We describe a rapid and convenient method of growing streptavidin (SA) monolayer crystals directly on holey-carbon EM grids. As expected, these SA monolayer crystals retain their biotin-binding function and crystalline order through a cycle of embedding in trehalose and, later, its removal. This fac...

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Detaylı Bibliyografya
Yayımlandı:J Struct Biol
Asıl Yazarlar: Han, Bong-Gyoon, Watson, Zoe, Kang, Hannah, Pulk, Arto, Downing, Kenneth H., Cate, Jamie, Glaeser, Robert M.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2016
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4943657/
https://ncbi.nlm.nih.gov/pubmed/27320699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2016.06.009
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