Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons

A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for cDNA synthesis and Nextera XT bar...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Τόπος έκδοσης:Nat Protoc
Κύριοι συγγραφείς: Krishnaswami, Suguna Rani, Grindberg, Rashel V, Novotny, Mark, Venepally, Pratap, Lacar, Benjamin, Bhutani, Kunal, Linker, Sara B, Pham, Son, Erwin, Jennifer A, Miller, Jeremy A, Hodge, Rebecca, McCarthy, James K, Kelder, Martin, McCorrison, Jamison, Aevermann, Brian D, Fuertes, Francisco Diez, Scheuermann, Richard H, Lee, Jun, Lein, Ed S, Schork, Nicholas, McConnell, Michael J, Gage, Fred H, Lasken, Roger S
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: 2016
Θέματα:
Article
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941947/
https://ncbi.nlm.nih.gov/pubmed/26890679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.015
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