Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons

A protocol is described for sequencing the transcriptome of a cell nucleus. Nuclei are isolated from specimens and sorted by FACS, cDNA libraries are constructed and RNA-seq is performed, followed by data analysis. Some steps follow published methods (Smart-seq2 for cDNA synthesis and Nextera XT bar...

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Publicado en:Nat Protoc
Main Authors: Krishnaswami, Suguna Rani, Grindberg, Rashel V, Novotny, Mark, Venepally, Pratap, Lacar, Benjamin, Bhutani, Kunal, Linker, Sara B, Pham, Son, Erwin, Jennifer A, Miller, Jeremy A, Hodge, Rebecca, McCarthy, James K, Kelder, Martin, McCorrison, Jamison, Aevermann, Brian D, Fuertes, Francisco Diez, Scheuermann, Richard H, Lee, Jun, Lein, Ed S, Schork, Nicholas, McConnell, Michael J, Gage, Fred H, Lasken, Roger S
Formato: Artigo
Idioma:Inglês
Publicado: 2016
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941947/
https://ncbi.nlm.nih.gov/pubmed/26890679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.015
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