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A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity

The precise role of the spliceosomal DEAD-box protein Prp28 in higher eukaryotes remains unclear. We show that stable tri-snRNP association during pre-catalytic spliceosomal B complex formation is blocked by a dominant-negative hPrp28 mutant lacking ATPase activity. Complexes formed in the presence...

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Détails bibliographiques
Publié dans:Nat Commun
Auteurs principaux: Boesler, Carsten, Rigo, Norbert, Anokhina, Maria M., Tauchert, Marcel J., Agafonov, Dmitry E., Kastner, Berthold, Urlaub, Henning, Ficner, Ralf, Will, Cindy L., Lührmann, Reinhard
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2016
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4935976/
https://ncbi.nlm.nih.gov/pubmed/27377154
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms11997
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