Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue fixation-embedding, detergent-clearing and staining protocols that can be used to transform excised organs and whole organisms into optically transparent samples within 1–2 weeks without compromising their cell...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Treweek, Jennifer B, Chan, Ken Y, Flytzanis, Nicholas C, Yang, Bin, Deverman, Benjamin E, Greenbaum, Alon, Lignell, Antti, Xiao, Cheng, Cai, Long, Ladinsky, Mark S, Bjorkman, Pamela J, Fowlkes, Charless C, Gradinaru, Viviana
Format: Artigo
Sprog:Inglês
Udgivet: 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4917295/
https://ncbi.nlm.nih.gov/pubmed/26492141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.122
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