Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue fixation-embedding, detergent-clearing and staining protocols that can be used to transform excised organs and whole organisms into optically transparent samples within 1–2 weeks without compromising their cell...

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Detaylı Bibliyografya
Yayımlandı:Nat Protoc
Asıl Yazarlar: Treweek, Jennifer B, Chan, Ken Y, Flytzanis, Nicholas C, Yang, Bin, Deverman, Benjamin E, Greenbaum, Alon, Lignell, Antti, Xiao, Cheng, Cai, Long, Ladinsky, Mark S, Bjorkman, Pamela J, Fowlkes, Charless C, Gradinaru, Viviana
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2015
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4917295/
https://ncbi.nlm.nih.gov/pubmed/26492141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.122
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