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Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue fixation-embedding, detergent-clearing and staining protocols that can be used to transform excised organs and whole organisms into optically transparent samples within 1–2 weeks without compromising their cell...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Treweek, Jennifer B, Chan, Ken Y, Flytzanis, Nicholas C, Yang, Bin, Deverman, Benjamin E, Greenbaum, Alon, Lignell, Antti, Xiao, Cheng, Cai, Long, Ladinsky, Mark S, Bjorkman, Pamela J, Fowlkes, Charless C, Gradinaru, Viviana
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4917295/
https://ncbi.nlm.nih.gov/pubmed/26492141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.122
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