Single-molecule RNA detection at depth by hybridization chain reaction and tissue hydrogel embedding and clearing

Accurate and robust detection of mRNA molecules in thick tissue samples can reveal gene expression patterns in single cells within their native environment. Preserving spatial relationships while accessing the transcriptome of selected cells is a crucial feature for advancing many biological areas –...

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Foilsithe in:Development
Main Authors: Shah, Sheel, Lubeck, Eric, Schwarzkopf, Maayan, He, Ting-Fang, Greenbaum, Alon, Sohn, Chang Ho, Lignell, Antti, Choi, Harry M. T., Gradinaru, Viviana, Pierce, Niles A., Cai, Long
Formáid: Artigo
Teanga:Inglês
Foilsithe: The Company of Biologists Ltd 2016
Ábhair:
Techniques and Resources
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5004914/
https://ncbi.nlm.nih.gov/pubmed/27342713
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.138560
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