Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue fixation-embedding, detergent-clearing and staining protocols that can be used to transform excised organs and whole organisms into optically transparent samples within 1–2 weeks without compromising their cell...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nat Protoc
Hauptverfasser: Treweek, Jennifer B, Chan, Ken Y, Flytzanis, Nicholas C, Yang, Bin, Deverman, Benjamin E, Greenbaum, Alon, Lignell, Antti, Xiao, Cheng, Cai, Long, Ladinsky, Mark S, Bjorkman, Pamela J, Fowlkes, Charless C, Gradinaru, Viviana
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
Schlagworte:
Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4917295/
https://ncbi.nlm.nih.gov/pubmed/26492141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.122
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge