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Reprogramming Caspase-7 Specificity by Regio-Specific Mutations and Selection Provides Alternate Solutions for Substrate Recognition

The ability to routinely engineer protease specificity can allow us to better understand and modulate their biology for expanded therapeutic and industrial applications. Here we report a new approach based on a caged green fluorescent protein (CA-GFP) reporter that allows for flow-cytometry-based se...

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Pubblicato in:ACS Chem Biol
Autori principali: Hill, Maureen E., MacPherson, Derek J., Wu, Peng, Julien, Olivier, Wells, James A., Hardy, Jeanne A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4912419/
https://ncbi.nlm.nih.gov/pubmed/27032039
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.5b00971
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