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Pre-Steady-State Kinetic Analysis of Single-Nucleotide Incorporation by DNA Polymerases
Pre-steady-state kinetic analysis is a powerful and widely used method to obtain multiple kinetic parameters. This protocol provides a step-by-step procedure for pre-steady-state kinetic analysis of single-nucleotide incorporation by a DNA polymerase. It describes the experimental details of DNA sub...
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| izdano v: | Curr Protoc Nucleic Acid Chem |
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| Main Authors: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2016
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4903026/ https://ncbi.nlm.nih.gov/pubmed/27248785 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpnc.2 |
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