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Steady-State Kinetic Analysis of DNA Polymerase Single-Nucleotide Incorporation Products
This unit describes the experimental procedures for the steady-state kinetic analysis of DNA synthesis across DNA nucleotides (native or modified) by DNA polymerases. In vitro primer extension experiments with a single nucleoside triphosphate species followed by denaturing polyacrylamide gel electro...
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| Publicado no: | Curr Protoc Nucleic Acid Chem |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4274652/ https://ncbi.nlm.nih.gov/pubmed/25501593 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142700.nc0721s59 |
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