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Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent
BACKGROUND: Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buf...
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| Publicado no: | Microb Cell Fact |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4898390/ https://ncbi.nlm.nih.gov/pubmed/27277580 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0504-9 |
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