Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

BACKGROUND: Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buf...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Microb Cell Fact
Hlavní autoři: Upadhyay, Vaibhav, Singh, Anupam, Jha, Divya, Singh, Akansha, Panda, Amulya K.
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2016
Témata:
Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4898390/
https://ncbi.nlm.nih.gov/pubmed/27277580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0504-9
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky