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Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein

A tetrameric protein of therapeutic importance, Escherichia coli L-asparaginase-II was expressed in Escherichia coli as inclusion bodies (IBs). Asparaginase IBs were solubilized using low concentration of urea and refolded into active tetrameric protein using pulsatile dilution method. Refolded aspa...

詳細記述

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書誌詳細
主要な著者: Upadhyay, Arun K., Singh, Anupam, Mukherjee, K. J., Panda, Amulya K.
フォーマット: Artigo
言語:Inglês
出版事項: Frontiers Media S.A. 2014
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4164012/
https://ncbi.nlm.nih.gov/pubmed/25309524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2014.00486
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