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Improved PCR Amplification of Broad Spectrum GC DNA Templates

Many applications in molecular biology can benefit from improved PCR amplification of DNA segments containing a wide range of GC content. Conventional PCR amplification of DNA sequences with regions of GC less than 30%, or higher than 70%, is complex due to secondary structures that block the DNA po...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Guido, Nicholas, Starostina, Elena, Leake, Devin, Saaem, Ishtiaq
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4896431/
https://ncbi.nlm.nih.gov/pubmed/27271574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0156478
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