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A Fundamental Study of the PCR Amplification of GC-Rich DNA Templates
A theoretical analysis is presented with experimental confirmation to conclusively demonstrate the critical role that annealing plays in efficient PCR amplification of GC-rich templates. The analysis is focused on the annealing of primers at alternative binding sites (competitive annealing) and the...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2727727/ https://ncbi.nlm.nih.gov/pubmed/18760969 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.compbiolchem.2008.07.021 |
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