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Gene synthesis by integrated polymerase chain assembly and PCR amplification using a high-speed thermocycler
Polymerase chain assembly (PCA) is a technique used to synthesize genes ranging from a few hundred base pairs to many kilobase pairs in length. In traditional PCA, equimolar concentrations of single stranded DNA oligonucleotides are repeatedly hybridized and extended by a polymerase enzyme into long...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3691701/ https://ncbi.nlm.nih.gov/pubmed/19799938 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2009.09.015 |
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