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Rational de novo Gene Synthesis by Rapid Polymerase Chain Assembly (PCA) and Expression of Endothelial Protein-C and Thrombin Receptor Genes
The assembly of synthetic oligonucleotides into genes and genomes is an important methodology. Several methodologies for such synthesis have been developed, but they have two drawbacks: 1) the processes are slow and 2) the error frequencies are high (typically 1 – 3 errors/kb of DNA). Thermal damage...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3347765/ https://ncbi.nlm.nih.gov/pubmed/17875333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jbiotec.2007.08.010 |
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