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Challenge of mimicking the influences of the cellular environment on RNA structure by PEG-induced macromolecular crowding

There are large differences between the cellular environment and the conditions widely used to study RNA in vitro. SHAPE RNA structure probing in Escherichia coli cells has shown that the cellular environment stabilizes both long-range and local tertiary interactions in the adenine riboswitch aptame...

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Publicado en:Biochemistry
Main Authors: Tyrrell, Jillian, Weeks, Kevin M., Pielak, Gary J.
Formato: Artigo
Idioma:Inglês
Publicado: 2015
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4893815/
https://ncbi.nlm.nih.gov/pubmed/26430778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b00767
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