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Challenge of mimicking the influences of the cellular environment on RNA structure by PEG-induced macromolecular crowding

There are large differences between the cellular environment and the conditions widely used to study RNA in vitro. SHAPE RNA structure probing in Escherichia coli cells has shown that the cellular environment stabilizes both long-range and local tertiary interactions in the adenine riboswitch aptame...

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Publicat a:Biochemistry
Autors principals: Tyrrell, Jillian, Weeks, Kevin M., Pielak, Gary J.
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4893815/
https://ncbi.nlm.nih.gov/pubmed/26430778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.5b00767
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