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Light-induced nuclear export reveals rapid dynamics of epigenetic modifications

We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylat...

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Bibliografiske detaljer
Udgivet i:Nat Chem Biol
Main Authors: Yumerefendi, Hayretin, Lerner, Andrew Michael, Zimmerman, Seth Parker, Hahn, Klaus, Bear, James E, Strahl, Brian D., Kuhlman, Brian
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4888063/
https://ncbi.nlm.nih.gov/pubmed/27089030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2068
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