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Stoichiometry and Affinity of Thioflavin T Binding to Sup35p Amyloid Fibrils

In this work two modes of binding of the fluorescent probe thioflavin T to yeast prion protein Sup35p amyloid fibrils were revealed by absorption spectrometry of solutions prepared by equilibrium microdialysis. These binding modes exhibited significant differences in binding affinity and stoichiomet...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Sulatskaya, Anna I., Kuznetsova, Irina M., Belousov, Mikhail V., Bondarev, Stanislav A., Zhouravleva, Galina A., Turoverov, Konstantin K.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4882037/
https://ncbi.nlm.nih.gov/pubmed/27228180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0156314
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