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Analyzing Thioflavin T Binding to Amyloid Fibrils by an Equilibrium Microdialysis-Based Technique

A new approach for the determination of the amyloid fibril – thioflavin T (ThT) binding parameters (the number of binding modes, stoichiometry, and binding constants of each mode) is proposed. This approach is based on the absorption spectroscopy determination of the concentration of free and bound...

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Détails bibliographiques
Auteurs principaux: Kuznetsova, Irina M., Sulatskaya, Anna I., Uversky, Vladimir N., Turoverov, Konstantin K.
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3286464/
https://ncbi.nlm.nih.gov/pubmed/22383971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0030724
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