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Analyzing Thioflavin T Binding to Amyloid Fibrils by an Equilibrium Microdialysis-Based Technique

A new approach for the determination of the amyloid fibril – thioflavin T (ThT) binding parameters (the number of binding modes, stoichiometry, and binding constants of each mode) is proposed. This approach is based on the absorption spectroscopy determination of the concentration of free and bound...

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Detalhes bibliográficos
Main Authors: Kuznetsova, Irina M., Sulatskaya, Anna I., Uversky, Vladimir N., Turoverov, Konstantin K.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3286464/
https://ncbi.nlm.nih.gov/pubmed/22383971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0030724
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