A novel non-radioactive assay for HIV-RT (RdDp) based on pyrosequencing for high-throughput drug screening

Current in vitro assays for the activity of HIV-RT (reverse transcriptase) require radio-labeled or chemically modified nucleotides to detect reaction products. However, these assays are inherently end-point measurements and labor intensive. Here we describe a novel non-radioactive assay based on th...

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Bibliographic Details
Published in:Protein Cell
Main Authors: Zhang, Chang, Wu, Yang, Sun, Yuna, Hong, Chuan, Xiang, Kehui, Guo, Yu, Bartlam, Mark, Lou, Zhiyong
Format: Artigo
Language:Inglês
Published: Higher Education Press 2010
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4875084/
https://ncbi.nlm.nih.gov/pubmed/21203975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13238-010-0031-0
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