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A novel non-radioactive assay for HIV-RT (RdDp) based on pyrosequencing for high-throughput drug screening
Current in vitro assays for the activity of HIV-RT (reverse transcriptase) require radio-labeled or chemically modified nucleotides to detect reaction products. However, these assays are inherently end-point measurements and labor intensive. Here we describe a novel non-radioactive assay based on th...
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Publicado no: | Protein Cell |
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Main Authors: | , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Higher Education Press
2010
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4875084/ https://ncbi.nlm.nih.gov/pubmed/21203975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13238-010-0031-0 |
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