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Optimization of a Non-Radioactive High-Throughput Assay for Decarboxylase Enzymes
Herein, we describe the optimization of a linked enzyme assay suitable for high-throughput screening of decarboxylases, a target family whose activity has historically been difficult to quantify. Our approach uses a commercially available bicarbonate detection reagent to measure decarboxylase activi...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Mary Ann Liebert, Inc. publishers
2010
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3098607/ https://ncbi.nlm.nih.gov/pubmed/20085486 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/adt.2009.0249 |
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