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Optimization of a Non-Radioactive High-Throughput Assay for Decarboxylase Enzymes

Herein, we describe the optimization of a linked enzyme assay suitable for high-throughput screening of decarboxylases, a target family whose activity has historically been difficult to quantify. Our approach uses a commercially available bicarbonate detection reagent to measure decarboxylase activi...

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Bibliografische gegevens
Hoofdauteurs: Smithson, David C., Shelat, Anang A., Baldwin, Jeffrey, Phillips, Margaret A., Guy, R. Kiplin
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Mary Ann Liebert, Inc. publishers 2010
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3098607/
https://ncbi.nlm.nih.gov/pubmed/20085486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/adt.2009.0249
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